week 11/18- 22

This week another gel was run on digested lambda and plain lambda for about 4 hrs and 30 mins; after there was concern that the gel did not run long enough for the ladder to show the higher base pairs (50k to 25k base pairs). However, after running the gel for so long, the plain showed 2 bands on the gel, which was confusing because you would expect that from the digested lambda. After talking to the professor, it was concluded I might have switched the tubes for the digested and not digested lambda. 

Recap on why we were experimenting with lambda DNA.

An issue we are having while trying to ligate kanamycin and PGRNA is that we confirmed previously that the x-bai site is not binding at all, so to diagnose what exactly in our process is not working correctly, we are using lambda as our control (comparing it to our plasmids). We are doing everything exactly the same way to make sure we catch what we were doing wrong. 

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