week 10/21-25

This week was mostly about gram-staining pd cas9 and learning to use the 4-way streak method for better colonies of growth on the agar plates. I learned it was best practice to streak the plate facing down for less risk of contamination, but I prefer to streak face up for more accuracy. 

    When gram-staining growth from these plates, in this case Pd Cas9, it's good to know that a little more time on the ethanol step won't harm the quality of the stain too much, especially if the cells are compacted. 

After these plates were gramstained, they were passaged onto R2A and TGY plates poured during the week. 

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