week 11/15-18

This week we started to explore the idea of using lambda (#N3011S from New England BioLabs) as a control group to figure out what exactly is going on with the ligation of PGK, and one issue we're hoping is the problem is the digestion of the XbaI site of the fragments. One of the most important things when using this method is to do everything exactly the same way, or else we wouldn't know exactly what is going wrong. 

Key information about lambda

  • Concentration: 500 µg/ml
  • Base Pairs: 48,502
  • XbaI Site: Located at 24,508 bp, leaving a 23,994 bp fragment after digestion.

    • Lambda was digested with x bai overnight and then run on a 2% 120 ml gel for around 2 hours. 

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