week 11/25 -27

This week we started on a new project with one of my labmates that is researching the transformation of pRAD 1
     Based on research, media with less nutrition (diluted) can contribute to a better yield of competent cells. 
       It was decided that to better test what works more effectively in making competent cells, a 3-factor experiment would best
        Factors
                Cells/ Plasmid Ratio 
                Temperature for transformation
                starvation media
The concentrations used for the of media (R2A)  were calculated by percentage 
    100% -- 1.360g 
    50% -- 0.68g 
    25% -- 0.34g 
    12.5% -- 0.17g 


A gel was then loaded to confirm that the plasmid is pRAd1
    45 mins
     run at 70vm

The gel looked really good but the latter could have been run for a little longer to further separate the bands 

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