First week of Bio tech Lab (9/3 to 9/4)

 The first few weeks of Lab consisted of mostly shadowing groups and taking a lot of notes about the nature of the lab and what kind of experiments are run during the week. Below is a list of things I learned or observed 

  • How an agarose gel is made for an electrophoresis machine which one of its uses is to run PCR products on to determine if transformation or ligation has occurred based on base pairs
  • How to load a gel and the procedure for preparing products for the gel like how much dye and how much PCR water needs to be used in order for the bands created to be visible 
  • Also on the first day I observed how some procedures for plasmid extraction is to cut out the band from the gel after it has ran on the electrophoresis machine. later on during lab I learned that using this technique might not be the best to use if you have a small amount because you lose around half of your sample or product 
  • E.coli grows in the 37 degrees Celsius incubator. 
  • How to make a concentration of Ampicillin and Media for agar plates.


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