week 11/25 -27
This week we started on a new project with one of my labmates that is researching the transformation of pRAD 1 Based on research, media with less nutrition (diluted) can contribute to a better yield of competent cells. It was decided that to better test what works more effectively in making competent cells, a 3-factor experiment would best Factors Cells/ Plasmid Ratio Temperature for transformation starvation media The concentrations used for the of media (R2A) were calculated by percentage 100% -- 1.360g 50% -- 0.68g 25% -- 0.34g 12.5% -- 0.17g A gel was then loaded to confirm th...